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文献和实验stimulation. In addition, application of cell based assays simultaneously permits entry level evaluation of compound toxicity and endows with a powerful approach to perform high-throughput screenings of, e.g., small molecule libraries in a quest for novel
Using Bioinformatics Tools for the Sequence Analysis of Immunoglobulins and T Cell Receptors
concepts, were the prerequisites for the implementation of the IMGT/V?QUEST and IMGT/JunctionAnalysis tools. IMGT/V?QUEST analyzes germline V and rearranged V?J or V?D?J nucleotide sequences. IMGT/JunctionAnalysis is the first tool that automatically
胶:1%~2% 的琼脂溶在 0.5X Tris-baseboric 酸-EDTA (TBE) 缓冲液。 ( 17 ) 10X TBE 缓冲液:1 mol/L Tris-base、1 mol/L boric 酸、20 mmol/L EDIA,pH 8.0。 ( 18 ) QiaexII 切胶回收试剂盒(Qiagen) 。 ( 19 ) dNTP (Amersham)。 ( 20 ) 水浴锅(Epperndorf)。 ( 21 ) MgCl2 储液:25~100 mmol/L
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