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- 应用范围:
Positive Control
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0
- 库存:
大量
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0
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0
- 规格:
50 μg
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Integrin alphaVbeta3 is purified and presented in 20mM Tris-HCI, pH 7.5, 150mM NaCl2, 2mM MgCl2 , 0.1mM CaCl2. 0.2% Triton X-100.
Optimal working dilutions must be determined by end user.
- ITGAV
- DKFZp686A08142
- MSK8
- CD51
- VNRA
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文献和实验Activation and Expansion of Human Treg Cells
of Human Treg Cells Day 0: Start with 1 x 105 Treg cells in 100 μl Culture Medium in a round bottom 96 well tissue culture plate. Add 20 μl Dynabeads Human Treg Expander. Add 500 U/ml rIL-2. Day 1: Add 100 μl Culture Medium containing 500 U/ml
Culture of Human Embryonic Stem Cells (hESC)
with 0.1% gelatin (Sigma Cat #G1890) in dH2 O. Mouse embryonic fibroblasts (CF1 strain), cultured in MEF medium , are mitotically inactivated by treatment with 10μg/ml mitomycin C (Roche Cat #107 409) for 2 to 3 hours at 37°C. Cells are washed
PriCells: Methods of Isolation and Culture of Human Hepatocytes
should be removed and rapidly frozen. The monolayer should be washed with phosphate-buffered saline (PBS). The hepatocyte monolayer can then be disrupted by either a detergent solution (Triton X-100, 1% solution in PBS) or mechanical action. The supernatant
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