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Goat anti-Rabbit IgG, Biotin-S

P conjugate; 2 mL
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  • 询价
  • AP132B
  • 2025年07月10日
  • ELISA;Western Blotting
  • Goat
  • Rabbit
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      ELISA;Western Blotting

    • 宿主

      Goat

    • 抗原来源

      0

    • 适应物种

      Rabbit

    • 库存

      大量

    • 是否单克隆

      2

    • 规格

      2 mL

    Species Reactivity Key Applications Host Format Antibody Type
    Rb ELISA, WB Goat Biotin Polyclonal Antibody
    Description:
    Goat anti-Rabbit IgG, biotin-SP conjugate
    Background Information:
    Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
    Key Applications:
    • ELISA
    • Western Blotting
    Application Notes:
    EIA: 1:50,000-1:100,000

    Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.

    Immunohistochemistry: 1:500-1:5,000

    Flow cytometry: 1:200-1:1,000

    Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000

    Optimal working dilutions must be determined by end user.
    Species Reactivity:
    Rabbit
    Presentation:
    RECONSTITUTION:

    Reconstitute with sterile distilled water to match the volume indicated on the vial label.
    Storage Conditions:
    Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

    WARNING:

    For research use only; not for use as a diagnostic.
    Antibody Category:
    Secondary & Control Antibodies
    Antibody Sub-Category:
    Whole Immunoglobulin Secondary Antibodies
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Biotin
    Host:
    Goat
    Product Overview:
    Affinity purified goat anti-rabbit IgG, Biotin conjugated.
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    详细描述见链接:http://www.millipore.com/catalogue/item/AP132B

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    图标文献和实验
    相关实验
    • Anti-P-CREB

      and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection

    • Anti-HA Immunocytology

      -purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)  

    • Anti-GFP/BrdU In Situ Immunostaining

      by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v,  plus 1% goat serum in PBS) and incubate 1 hr. at room temp.  Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp.  Wash 3x in PBS. Denature DNA with 1 mL

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