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Chemicon(Millipore) Goat anti-

Mouse IgG Biotin-SP conjugate ELISA Western Blotting
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  • 询价
  • AP124B
  • 2026年01月08日
  • ELISA;Western Blotting
  • Goat
  • Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      ELISA;Western Blotting

    • 宿主：

      Goat

    • 适应物种：

      Mouse

    • 抗原来源：

      0

    • 库存：

      大量

    • 是否单克隆：

      2

    • 规格：

      2 mL

    Species Reactivity Key Applications Host Format Antibody Type
    M ELISA, WB Goat Biotin Polyclonal Antibody
    Description:
    Goat anti-Mouse IgG, biotin-SP conjugate
    Background Information:
    Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
    Key Applications:
    • ELISA
    • Western Blotting
    Application Notes:
    EIA: 1:50,000-1:100,000

    Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.

    Immunohistochemistry: 1:500-1:5,000

    Flow cytometry: 1:200-1:1,000

    Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000

    Optimal working dilutions must be determined by end user.
    Species Reactivity:
    Mouse
    Presentation:
    PRESENTATION: Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.1% sodium azide.

    RECONSTITUTION:

    Reconstitute with sterile distilled water to match the volume indicated on the vial label.
    Storage Conditions:
    Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
    Antibody Category:
    Secondary & Control Antibodies
    Antibody Sub-Category:
    Whole Immunoglobulin Secondary Antibodies
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Biotin
    Host:
    Goat
    Product Overview:
    Affinity purified goat anti-mouse IgG, Biotin conjugated.
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    详细描述见链接：http://www.millipore.com/catalogue/item/AP124B

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    图标文献和实验
    相关实验

    • （共享）ELISA原理与实验方法

      , incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

    • 【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

      : Total mouse IgG Ig Type: mouse IgG specific goat IgG Applications: Western blot ELISA Preparation Produced in goats immunized with purified total mouse IgG. Mouse IgG1, mouse IgG2A, mouse IgG2B and mouse IgG3 specific IgG was purified by mouse IgG

    • Western Blotting Protocols

      or overnight at 4°C with agitation. For a mouse monoclonal antibody, a goat anti-mouse HRP-conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat anti-rabbit HRP-conjugated antibody is advisable. Wash the membrane five times

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