- ￥2433
- Sigma-Aldrich
- 进口
- R5503-500MG
- 2026年02月01日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
−20°C
- 保质期：
根据瓶身LOT号查询
- 英文名：
Ribonuclease A from bovine pancreas
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
9001-99-4
- 规格：
500MG
属性
生物来源
bovine pancreas
质量水平
300
类型
Type I-AS
形式
lyophilized powder
比活
50-100 Kunitz units/mg protein
分子量
~13,700
技术
cell based assay: suitable
杂质
salt, essentially free
适用性
suitable for mRNA or total RNA extracted from cells and tissues
应用
diagnostic assay manufacturing
异质活性
protease, essentially free
储存温度
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
一般描述
应用
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
生化/生理作用
特点和优势
制备说明
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文献和实验Proteomics Links Ubiquitin Chain Topology Change to Transcription Factor Activation.
A surprising complexity of ubiquitin signaling has emerged with identification of different ubiquitin chain topologies. However, mechanisms of how the diverse ubiquitin codes control biological processes remain poorly understood. Here, we use quantitative whole-proteome mass spectrometry to identify yeast proteins that are regulated by lysine 11 (K11)-linked ubiquitin chains. The entire Met4 pathway, which links cell proliferation with sulfur amino acid metabolism, was significantly affected by K11 chains and selected for mechanistic studies. Previously, we demonstrated that a K48-linked ubiquitin chain represses the transcription factor Met4. Here, we show that efficient Met4 activation requires a K11-linked topology. Mechanistically, our results propose that the K48 chain binds to a topology-selective tandem ubiquitin binding region in Met4 and competes with binding of the basal transcription machinery to the same region. The change to K11-enriched chain architecture releases this competition and permits binding of the basal transcription complex to activate transcription.
：Formerly listed as Type III 溶解性：溶于水，参考浓度2mg/ml 储存条件：2-8℃ From Sigma D1626 Salmon DNA 的配制： 组份浓度10 mg/ml Salmon DNA ;配制量约100 ml 配制方法: 1. 称取鲑鱼精DNA 2 g置于500 ml烧杯中，加入约200 ml的TE Buffer。 2. 用磁力搅拌器室温搅拌2～4小时
, although some hypotheseshave been suggested. The first hypothesis proposes lithium as amagnesium (Mg 2+ )-competitive inhibition of GSK-3 (16) , whereasthe second suggests lithium inhibitor by potassium deprivation (17) . Lithium is a nonspecific GSK-3 inhibitor
% 戊二醛 0.1ml ， 37 ℃ 温育 2h 后加入冰冷的分析纯的无水乙醇 2ml ， 2 500r/min 离心沉淀 10min~15min ，倾去溶液。沉淀以 80% 乙醇 4ml~5ml 混悬，同上离心，倾去乙醇，将管倒置，使乙醇充分流出。沉淀用 1ml 0.05Mol/L pH9.6 碳酸盐缓冲液溶解，加入 0.5ml~1ml 抗 IgG 抗体溶液 ( 含抗体 15mg 左右 ) ，放在冰箱内过夜后，加 KH2 PO4 ，使近中性即可应用。
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