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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
−20°C
- 保质期:
根据瓶身LOT号查询
- 英文名:
Guanosine 5′-triphosphate sodium salt hydrate
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
36051-31-7
- 规格:
10MG
属性
质量水平
200
方案
≥95% (HPLC)
表单
powder
颜色
white
溶解性
H2O: 50 mg/mL
运输
dry ice
储存温度
−20°C
SMILES字符串
[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
InChI
1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1
InChI key
KZRMTEVIDYXWQW-CYCLDIHTSA-K
基因信息
human ... HRAS(3265)
应用
- 辣根过氧化物酶(HRP)修饰的平板试剂制备
- 全细胞电压钳记录
- 转谷氨酰胺酶(tTG)的体外抑制
生化/生理作用
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文献和实验Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation.
Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones. Determining the molecular size of the importin-histone complexes is key to understanding the mechanism of histone transport and also the potential roles of importins as histone chaperones and in the assembly of nucleosomes. Here we report a simple and reproducible sedimentation-velocity based method to determine the molecular sizes of importin-histone complexes using analytical ultracentrifugation. The method does not use any reporter tags or interaction with column resin thereby analyzing the interactions of the native proteins.
核酸及蛋白质常用数据1.核苷三磷酸的物理常数化合物分子量λmax(pH7.0)1摩尔溶液(pH7.0)中λmax时的最大吸收值OD280/OD260ATP507259154000.15CTP48327190000.97GTP523253137000.66UTP484262100000.38dATP494259152000.15dCTP46727193000.98dGTP507253137000.66dTTP48226796000.712.常用核酸的长度与分子量核酸核苷酸数分子量λDNA
酮酸(nalidixicacid)(5mg/ml) 溶解0.05g萘啶酮酸钠盐于足量的水中,最后定容至10ml。分装成小份于-20℃贮存。常以15ug/ml的终浓度添加于生长培养基。 四环素(tetracyyline)(10mg/ml) 溶解0.1g四环素盐酸盐于足量的水中,或者将无碱的四环素溶于无水乙醇,定容至10ml。分装成小份用铝箔包裹装液管以免溶液见光,于-20℃贮存。常以10ug/ml~50ug/ml的终浓度添加于生长培养基。
Recipe for yeast tryptone medium
bromide (EtBr) Contributor: Zappe, H Related: many protocols Care: never breath dust - mutagen Ethidium bromide (EtBr) Component stock conc final conc. / 10 ml /1l EtBr - 10mg/ml 0.1g water - - to 10
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