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- 保存条件:
常温
- 保质期:
根据瓶身LOT号查询
- 英文名:
Silicone oil
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
63148-62-9
- 规格:
250ML
属性
蒸汽密度
>1 (vs air)
质量水平
200
蒸汽压
<5 mmHg ( 25 °C)
5 mmHg ( 20 °C)
表单
viscous liquid
折射率
n20/D 1.403 (lit.)
粘度
500 cSt(25 °C)
沸点
>140 °C/0.002 mmHg (lit.)
密度
0.97 g/mL at 25 °C
一般描述
应用
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文献和实验Reverse engineering life: physical and chemical mimetics for controlled stem cell differentiation into cardiomyocytes.
Our ability to manipulate stem cells in order to induce differentiation along a desired developmental pathway has improved immeasurably in recent years. That is in part because we have a better understanding of the intracellular and extracellular signals that regulate differentiation. However, there has also been a realization that stem cell differentiation is not regulated only by chemical signals but also by the physical milieu in which a particular stem cell exists. In this regard we are challenged to mimic both chemical and physical environments. Herein we describe a method to induce stem cell differentiation into cardiomyocytes using a combination of chemical and physical cues. This method can be applied to produce differentiated cells for research and potentially for cell-based therapy of cardiomyopathies.
7.4的0.01mol/L的PBS液。4℃贮放备用。 (2)2—cis( )3 H—ABA液(干粉),3瓶(0.2μCi/瓶)。每瓶干粉加3.5ml PBS液溶解,每50μl含有7000─9000cpm(计数率62%)。避光,-20℃保存备用。 (3)2─cis( )ABA标准液(干粉),5瓶。每瓶加1ml PBS液溶解,每50μl分别含有300、600、1200、2400、4800pg ABA。避光,-20℃保存备用。 (4)兔抗2─cis(±)ABA抗体
,培养皿(120mm),脱脂棉,棕色试剂瓶,吸液管,吸耳球,玻璃纸,坐标纸。 2.试剂: 重蒸水,丙烯酰胺(重结晶),甲叉双丙烯酰胺(重结晶),过硫酸铵,TEMED(四甲基乙二胺),载体两性电解质(pH3.5~10),考马氏亮蓝R250,液体石腊,乙醇(95%),硅油,磺基水杨酸,三氯乙酸,甲醇,乙酸(36%),已知pI蛋白质样品和标准等电聚焦样品(市售),磷酸,氢氧化钠。 3.溶液配制: (1)单体贮液:丙烯酰胺2.91g,甲叉双丙烯酰胺0.9g,重蒸水溶解,定容到100ml,过滤
) (store at 4deg.C). IPTG (isopropyl b-D-thiogalactopyranoside):25 mg/ml IPTG in double distilled water 250 mg IPTG (Sigma I-5502) ddH2 O to 10 ml (aliquot and store at -20℃) 1 M isocitrate (sodium salt-dihydrate) : 29.41 g Na3isocitrate-2H
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