A peptide derived from human RapGEF1 around the phosphorylation site of Tyr504. Immunogen sequence location: 470-519
亚型:
Rabbit
形态:
Lyophilized
应用范围:
WB
保存条件:
低温冷藏
浓度:
0.5-1mg/ml
规格:
详见说明书
检验物种: human 应用范围: WB 产品类型: Polyclonal 产品名称 Anti-Phospho RAPGEF1 Antibody 基因名: RAPGEF1 抗体来源: Rabbit 克隆: Polyclonal 抗体亚型: Rabbit 免疫原: A peptide derived from human RapGEF1 around the phosphorylation site of Tyr504. Immunogen sequence location: 470-519 内容: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. 纯化方式: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. 浓度: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. 产品形态: Liquid 保存条件: 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. 实验步骤: ①滴加0.01mol/L,pH7.4的PBS于待检标本片上,10min后弃去,使标本保持一定湿度。 ②滴加适当稀释的荧光标记的抗体溶液,使其完全覆盖标本,置于有盖搪瓷盒内,保温一定时间(参考:30min)。 ③取出玻片,置玻片架上,先用0.01mol/L,pH7.4的PBS冲洗后,再按顺序过0.01mol/L,pH7.4的PBS三缸浸泡,每缸3-5 min,不时振荡。 ④取出玻片,用滤纸吸去多余水分,但不使标本干燥,加一滴缓冲甘油,以盖玻片覆盖。 ⑤立即用荧光显微镜观察。观察标本的特异性荧光强度,一般可用“+”表示: (-)无荧光;(±)极弱的可疑荧光;(+)荧光较弱,但清楚可见;(++)荧光明亮;(+++ --++++)荧光闪亮。待检标本特异性荧光染色强度达“++”以上,而各种对照显示为(±)或(-),即可判定为阳性。 公司专业供应的抗体,抗体是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品,Anti-Phospho RAPGEF1 Antibody品质卓越,价格实惠,多种规格供应,售后完善。