Anti-BACE C-terminus 兔多克隆抗体 人源

Anti-BACE, C-terminus

Alzheimer's disease (AD) is characterized by the progressive formation in the brain of insoluble amyloid plaques and vascular deposits consisting of the 4-kD amyloid b-peptide (Ab). Ab generation is initiated by proteolytic cleavage of the amyloid precursor protein (APP) at the N-terminal of Ab by b-secretase. The Ab peptide is then released by proteolytic cleavage at its C-terminus by g-secretase. Because both these proteases are prime candidates for therapeutic intervention, an intense search has been underway to identify these two enzymes.A human transmembrane aspartic-protease (Asp2), referred to as BACE, has been characterized and shown to have all the properties of b-secretase. Four groups in all have now confirmed that BACE (or Asp2) is a convincing candidate for b-secretase.

BACE is an N-glycosylated integral membrane aspartyl protease with Mr=70 kDa. Mature BACE is produced from the immature form through a series of post-translational proteolytic cleavages and glycosylation. Sequence analysis has revealed that the immature form of BACE contains an N-terminal signal sequence (residues 1-21) followed by a large catalytic domain, a single transmembrane domain (residues 461-477), and a short cytoplasmic domain (residues 478-501). The signal sequence (1-21) is cleaved from the immature form by a signal peptidase located in the endoplasmic reticulum (ER), yielding the proBACE protein (Mr=75 kDa) which starts at residue 22. The proBACE protein is modified by cleavage of 24 N-terminal residues (aa 22-45), producing the mature BACE protein.

ASP2; BACE1; Beta Secretase; beta-Site APP Cleaving Enzyme

BACE (beta-site APP Cleaving Enzyme).

• Western Blotting
• Immunohistochemistry

Western blot on COS7 transfected cells. The antibody recognizes a band of ~70-75 kDa. It also recognizes a band at ~45 kDa.

Immunohistochemistry on rat and monkey.

Optimal working dilutions must be determined by end user.

Human

Synthetic peptide corresponding to the C-terminus of human BACE.

C-terminus

70 kDa

Tested on human brain tissue lysate.

Purified Antibody in PBS with 0.1% NaN3

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

• BACE1
• ASP2
• beta-secretase
• Memapsin-2
• BACE
• memapsin-2
• KIAA1149
• HSPC104
• FLJ90568
• EC 3.4.23.46

Neuroscience

Neurodegenerative Diseases

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chemicon (Millipore)

Affinity Purified

Rabbit

Polyclonal Antibody