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描述 Nanosep centrifugal devices are formed out of two components, a filtrate receiver tube and a sample reservoir containing the membrane. These component parts are constructed from low protein binding polypropylene that also offers chemical compatibility for aggressive solvents. Instead of using O-rings or adhesives that could add extractables or bind precious biomolecules the product is constructed using an ultrasonic welding technique. These centrifugal devices are very simple to use. The sample to be processed is pipetted into the upper reservoir of the device. The device is placed into the appropriate centrifuge (taking care that the rotor is balanced) and spun. Filtrate can be recovered by removing the lower reservoir of the device and using a pipette or pipette tip to remove the liquid. Nanosep centrifugal devices can be used with sample volumes of up to 500 µL, and fit in standard centrifuge rotors that accept 1.5 mL tubes. Ensures rapid processing of samples Low protein binding Omega membrane for ultrafiltration applications A wide range of MWCOs, color coded for easy identification Constructed of low-binding polypropylene Ultrasonically welded seals prevent bypass or seal failure Fits standard centrifuge rotors that accept 1.5 mL tubes Nanosep centrifugal devices with Omega membranes can be used in a number of ultrafiltration applications including; the concentration, purification, and desalting of peptides, proteins, oligonucleotides, DNA, and RNA. They can also be used in the isolation of DNA from agarose gel slices, and to separate proteins, oligonucleotides, and RNA from acrylamide gels. Omega ultrafiltration membranes are manufactured from low protein binding modified polyethersulfone minimizing the possibility of sample losses due to non-specific binding. A wide selection of molecular weight cut-off (MWCO) options are available and devices are color coded for easy membrane selection. Pall supply users with a Nanosep concentration selection guide. The guide allows users to concentrate a sample to the concentration of their choice. This is achieved by selecting a volume of buffer solution to place in the device filtrate receiver tube prior to sample processing. The total volume of liquid in the device determines the final retentate volume. 类型 自旋装置 用途 样品浓度 脱盐 缓冲液更换
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文献和实验I buffer (Tris pH8.4, 20 mM MgCl2 , 500 mM KCl) 5 µL DNAse I (RNAse free) q.s. 100 µL with DEPC H2 O Incubate at room temp. x10 min. Immediately proceed to next step. RNeasy Mini Prep Procedure
制品的湿润性。另一个常用的方法是湿化学处理。这种处理方法可以通过浸泡在铬酸和磺酸的混合酸中以解决塑料表面的氧化部分。16电晕放电处理和铬酸处理的效果 为了说明电晕放电和铬酸对固体基底表面底处理效果,对用这些技术处理的六种工业塑料进行了一项研究用。电晕放电处理是将每一个塑料板的表面暴露在频率约有500kHz,电压为10000-50000v的放电电场中约5 秒钟。铬酸处理方法要求塑料板的表面用铬酸冲洗15秒钟,然后用蒸馏水冲洗干净。 再用异丙醇冲洗表面,然后擦干。以上每一种方法中,在处理完材料后应立即测量
Procedures for In Vitro Production of Bovine Embryos
have been washed, the contents are poured into an Intergrid plate and examined under a dissecting scope for COCs. These can be picked up using a wiretrol or other device.12. Collect oocyte cumulus complexes (COC) as fast as possible to prevent adverse effects
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