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描述 Microsep Advance centrifugal devices provide the rapid and efficient concentration and purification of up to 5.0 mL of biological samples. There unique design maximizes filtration area to process samples quickly while maintaining a gentle concentration environment to preserve protein activity and conformation. Achieve 50X concentration and > 90% recovery in just minutes Features deadstop to prevent samples from spinning to dryness Versatile ultrafiltration Omega membrane is available in a variety of MWCOs Color coded and laser etched for easy identification Omega ultrafiltration membranes are manufactured from low protein binding modified polyethersulfone minimizing the possibility of sample losses due to non-specific binding. A wide selection of molecular weight cut-off (MWCO) options are available and are color coded for easy membrane selection. Ultrafiltration devices are ideal for concentrating small peptides, oligonucleotides, nucleic acids, enzymes, antibodies, and other similar macromolecules. Microsep Advance centrifugal devices offer high recoveries, typically > 90%, the products feature a built-in deadstop that prevents spinning a sample to dryness. Pall supply users with a Microsep concentration selection guide. This guide allows users to concentrate a sample to the exact concentration of their choosing by selecting a volume of buffer solution to place in the devices filtrate receiver prior to sample processing. The total volume of liquid in the device determines the final retentate volume. Pall also supply Microsep Advance centrifugal devices with 0.2 and 0.45 μm pore sizes for rapid microfiltration applications. Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520. 应用 Ultrafiltration Concentrate dilute protein samples prior to electrophoresis Exchange buffer and remove salt in samples Isolate low molecular weight compounds from fermentation broths for natural product screening Recover biomolecules from cell culture supernatants or lysates Microfiltration Clarify samples with gross particulate Remove particulate from samples for HPLC analysis of drugs, amino acids, and antibodies 类型 自旋装置 用途 样品浓度 脱盐 缓冲液更换
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文献和实验Filter Binding Assay for EBNA-1
, 3ml syringePre-incubate filter with 100ul Binding Buffer at filter binding temperature (4C) until ready to bind DNA/EBNA-1 solution (e.g. conduct in 7th floor cold room).Incubate DNA fragment of interest with DNA with no known binding sites in a total
Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml. Autoclave. 1. Rock bottle gently to make a uniform slurry. 2. Place a disposable 3ml chromatography column into a 15 ml
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