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- STEMCELL Technologies
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- 2026年04月26日
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概要
DNA Loading Dye is a ready-to-use marker dye containing orange G (0.4%), bromophenol blue (0.03%), and xylene cyanol FF (0.03%), in 15% Ficoll® 400, 10 mM Tris-HCl (pH 7.5), and 50 mM EDTA (pH 8.0). The dye is used for loading DNA samples into agarose gel wells and tracking migration during electrophoresis. In a 0.5 - 1.4% agarose gel in 0.5X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp, and orange G at approximately 50 bp. This product is free of nuclease activity.
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文献和实验DNA sequencing (dye terminator)
Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/Big%20Dye%20Protocol.pdf
Sequencing off Bacterial Genomic DNA with ABI Big Dye Terminators
The following protocol and cycle conditions courtesy of Cheryl Heiner-ABI--------------------------------------------------------------------------------Protocol:Big Dye Terminator Mix* 16μlThermoFidelase** 1μlPrimer (18-24mer)15-30pmolesGenomic DNA
Over the past decade, fluorescent end-labeling of DNA fragments has evolved into the preferred method of DNA detection for a wide variety of applications, including DNA sequencing and polymerase chain reaction (PCR) fragment analysis
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