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件
Volume Ranges |
0.8 mL - 2.4 mL (0.8, 1.6, 2 & 2.4 mL) | |||||||||||||||
Performance |
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Dimensions |
8 in. or 20.32 cm. Length .795 in. or 2.02 cm. handle diameter |
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Weight |
1.0 oz. or 31.59 gm (instrument only) 9.8 oz. or 279 gm (Shipping weight) |
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Appearance |
Tan Body and Knob Gray Body and Teal Knob Black Hot Stamping |
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Fluid Contact Materials |
Polypropylene Glass Teflon? Platinum Iridium |
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Contents |
500 mL Plastic Bottle w/ 28 mm cap assembly (includes bottle inlet tubing & luer fitting) Three Volume Calibration Rings 30 in. Tygon? Remote Inlet Tubing Instructions |
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Accessories |
8-port manifolds - non sterile/disposable |
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文献和实验Dual- and Triple-Color Fluorospot
of spots a. Discard the cells and wash the plate five times with PBS in a plate washer (or by hand 200 μl/well; seeNote 7). b. In the same tube, dilute the detection mAbs for IFN-γ and IL-2 labeled with FITC and biotin, respectively
by filling wells with either a squirt bottle, carboy, manifold dispenser, multi- channel pipettor or automatic plate washer. For increased stringency, after each wash, let the plate stand briefly, flick off the buffer, and blot plates on paper towels
Transformation of E. coli by Electroporation
for 15 min and pour off the supernate. 6. Resuspend the cell pellet in WB to a final volume of 1 ml. Usually no additional WB needs to be added to the cell pellet; it can be resuspended in the WB that remains in the centrifuge bottle. Cells
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