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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human CD147 protein
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX02108
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
CD147
- 抗体英文名:
CD147 antibody [125]
- 抗体名:
CD147 抗体 [125]
- 规格:
100 μl
IHC-Fr analysis of monkey stomach tissue section using GTX02108 CD147 antibody [125].Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
IHC-P analysis of human esophagus tissue section using GTX02108 CD147 antibody [125].
Dilution : 1:5000
FACS analysis of HeLa cells using GTX02108 CD147 antibody [125].
IHC-P analysis of human placenta tissue section using GTX02108 CD147 antibody [125].
Dilution : 1:5000
IP analysis of K562 whole cell lysate using GTX02108 CD147 antibody [125].
IP antibody : 2 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
50 % Protein G agarose : 15 μl
IHC-P analysis of human rectal cancer tissue section using GTX02108 CD147 antibody [125].
Dilution : 1:5000
WB analysis of A431 whole cell lysate (Lane A) using GTX02108 CD147 antibody [125].
Dilution : 1:500
Loading : 30 μg
IHC-P analysis of human gastric cancer tissue section using GTX02108 CD147 antibody [125].
Dilution : 1:5000
IHC-P analysis of human liver tissue section using GTX02108 CD147 antibody [125].
Dilution : 1:5000
ICC/IF analysis of PFA-fixed HeLa cells using GTX02108 CD147 antibody [125].
Green : Primary antibody
Blue : DAPI
Dilution : 1:200
IHC-P analysis of human stomach tissue section using GTX02108 CD147 antibody [125].
Dilution : 1:5000
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文献和实验sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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