Falcon 5mL Round Bottom Polyst

Cell Cultures

from the cultures. Place the sample in a separate test tube (it need not be sterile). 2. Dilute 4 parts of stock Trypan Blue with 1 part of 5X saline and add 0.1 ml of the diluted dye to your sample. Mix gently. 3. Set up a hemocytometer and cover slip

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

coating with polymer or adding a layer of metal. Place the slide into a sterile rectangular four-chamber cell culture vessel (or use tissue culture slide flask if polystyrene surface is desired). Count cells and seed 1.5 x 106 cells onto the slide in 5 ml