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上海觅拓生物
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文献和实验High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
of propidium iodide (PI) fluorescence analysis and cell counting performed by the flow cytometer. Only 2 µl is aspirated from a culture for analysis so that assays can be performed in small volumes to minimize reagent cost and usage.
The Use of Propidium Iodide to Assess Excitotoxic Neuronal Death in Primary Mixed Cortical Cultures
in a variety of these pathologies. A key to testing such drugs is the use of a fast, reliable, and easily reproducible model of neurodegeneration and neuroprotection. Our laboratory has previously used propidium iodide (PI) to assess
(50 μg/mL PI, 0.1%柠檬酸钠, 0.1% Triton X-100), 以FACS检测细胞核DNA中亚二倍体的凋亡DNA波峰. 1)冰乙醇固定后无需穿孔? 2)低渗PI染液中Triton用量是多少?有的是100染液中用1%Triton 0.5ml。对吗? 3)配方: 柠檬酸三钠 0.25g Triton-X 100 0.75ml Propidium iodide 0.025g 核糖核酸酶 0.005g 蒸馏水 250ml PI
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