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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
上海觅拓生物
- 规格:
10 tablets
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文献和实验is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
ELISA PROTOCOL 【Yale University 】
-nitrophenyl phosphate (5 mg/tablet). SigmaCat.# N93895) AP-conjugated secondary Ab: AP-Rabbit anti-mouse IgG(Zymed). Reconstitute in ddH2O as prescribed by manufacturer andaliquot and store at -70 °C.6) 100 mM EDTA pH
DETECTION OF RECOMBINANT PROTEINS BY ELISA
2CO 3 slowly while stirring. Adjust pH to 8.6 and QS to 100 ml with H 2O. Substrate Solution (1 mg/ml paranitrophenyl phosphate PNPP) 100 mg capsule of PNPP (Sigma 104-100) 100 ml substrate buffer
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