Improved General Phosphatase I
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
EGTA stock, pH 8.0) in 100 ml PBS, pH 7.2-7.4 Heat about 80ml H2 O to 600 C and add paraformaldehyde; add 1-2 drops of 10M NaOH to get paraformaldehyde in solution. Cool to room temperature, add 10 ml 10X PBS, adjust pH with HCl, add MgCl
General Principles of Immunoprecipitation
.We usually use 2 mmolar.Sodium vanadate inhibits all tyrosine protein phosphatases.We use 200 micromolar and add it fresh for each experiment from a stock made in water and stored in a plastic tube at room temperature.Sodim fluoride is an inhibitor of Serine
100x Pen-Strep (P4333 or P0781) • 5 ml 100x sodium pyruvate (S8636 or P4562) • 5 ml beta-mercaptoethanol Stock solution = 3.5 µl diluted to 5 ml neat beta-maerpactoethanol (M7522) with tissue culture grade water (W3500) — make
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