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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD8
- 亚型:
mouse IgG2aκ
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
BW135/80
- 标记物:
APC
- 适应物种:
human, non-human primate
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
mouse
- 应用范围:
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunocytochemistry
- 浓度:
1:50
- 靶点:
CD8
- 抗体英文名:
CD8 Antibody, anti-human
- 抗体名:
CD8 Antibody, anti-human
- 规格:
100 tests in 200 µL
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文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
浓度为 1x107cells/ 100 μl,从中取 1 - 2x106 cells; 2. 用去离子水将 10xBuffer IS 稀释为 1x,准备 3 ml 稀释后的 Buffer IS; 3.取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC,0.8μl MHC I-Strep,与 1x Buffer IS 混匀为 10ul 的终体积,冰上孵育 45 分钟; 4.将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀,冰上孵育 45 分钟
Isolation of Untouched Human CD8+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)
at 1 x 108 PBMC per ml in Isolation buffer. 3. Isolation Procedure 1) Transfer 500 μl (5 × 107 ) PBMC in Isolation Buffer to a tube. 2) Add 100 μl heat inactivated FCS. 3) Add 100 μl of Antibody
ELISA Procedure for Measuring Serum Antibody Titer
Dilution ScheduleDilution ofAntiserum Volume of 1% BSA/PBS-T (µL) Volume of PreviousDilution (µL) 1:100 1000 1000 of 1:50 1:200 1000 1000 of 1:100 1:400 1000 1000 of 1:200 1:800 1000 1000 of 1:400 ... ... ... 1:102,400 1000 1000 of 1:51,200 11. Vortex
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