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- 2025年12月18日
- Protein Extraction
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Protein Extraction
- 宿主：
0
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
0
- 规格：
100 assays
This kit is recommended for use with CHEMICON®'s Rac Activation Assay Kit (Catalog No. SGT425), Cdc42 Activation Assay Kit (Catalog No. SGT430), Ras Activation Assay Kit (Catalog No. SGT435), and CHEMICON®'s Transcription Factor Assays when purified nuclear extracts are not required.
For Research Use Only; Not for use in diagnostic procedures
- Whole Cell Extraction Buffer, 5x: - (Part No. 90493) Two vials containing 10 mL of 5x concentrated buffer. Dilute to 1x final concentration with ddH2O prior to use.
- Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cells and tissue extracts. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators. Prior to use, dilute 1/1000 in 1x Whole Cell Extraction Buffer.
· The prepared 1x Whole Cell Extraction Buffer (with optional additives) should be stored at -20°C for up to one month.
· The undiluted Protease Inhibitor Cocktail can be stored at -20°C until the expiration date.
2. Cell Detachment Buffer (Trypsin) or Cell Scrapers
3. Phosphatase Inhibitors (if desired)
4. Syringes, 1mL - 27 Gauge Needle
5. 1.5 mL Microcentrifuge tubes
6. Deionized Water
7. Table-top Microcentrifuge, 4oC
8. Phosphate-Buffered Saline (PBS), Tris-Buffered Saline (TBS), or HBSS (Hank's Balanced Salt Solution)
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详细描述见链接：http://www.millipore.com/catalogue/item/2910
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文献和实验CellTrace CFSE Cell Proliferation Kit
实验材料 Kit Contents CellTrace™ CFSE (Component A), 10 vials, each containing 50 μg of lyophilized powder DMSO (Component B), 1 vial containing 0.5 mL of high quality dimethylsulfoxide
The Expanding Tool Kit for Hematopoietic Stem Cell Research
on a combination of cell-surface phenotype and functional characteristics. In addition, in vitro assays have been established that provide important tools for study of hematopoietic differentiation from HSC and for differentiation to generate HSC from embryonic
Dynabeads® Co-Immunoprecipitation Kit
scale of the cell sample recommended. For low abundant proteins, use the upper scale of the cell sample recommended. 2. Co-Immunoprecipitation Buffer System The co-immunoprecipitation buffer system provided in this kit
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