- 询价
- 2150
- 2025年12月17日
- Protein Clean-up
- 0
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Protein Clean-up
- 宿主：
0
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
0
- 规格：
10 ea
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文献和实验Isolation of micro-RNA (miRNA)
at 13�14k rpm for 5 minutes Transfer aqueous layer to a tube containing a volume equal to volume D of isopropanol. For samples less than 10 mg or 1 × 106 cells, mix 1 μl of glycogen with the RNA solution before adding the isopropanol. Spin
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
-through. Then with the collection tube empty, centrifuge the spin cartridge at 10,000 x g for 2 min at room temperature to completely dry the HiBind® matrix. 11. Transfer the column to a clean 1.5 ml micro centrifuge tube (not supplied with kit) and elute the RNA with 30-50
in the microfuge for 10 minutes at 14,000 rpm. Remove as much liquid as possible and add another 500 microliters of crush and soak solution. • Repeat the spin and pool the recovered supernatant. • Add 0.1 volume of 3M NaOAc, 2.5 volumes of EtOH and carrier
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