Strep-Tactin® Magnetic Microbe

Strep-Tactin^® Magnetic Microbeads are microspheres covalently linked to Strep-Tactin^®. They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer^®) or surface marker-specific cells (Fab-TACS^®/Nano-TACS^®). To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag^® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin^®. Strep-Tactin^® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.

Specifications

Bead Size:	1 - 3 µm
Form:	Suspension in DPBS
Possible Application:	Cell isolation (magnetic)
Recommended Usage:	15 µl per 1 x 10^7 total cells

Shipping information

Storage:	2-8 °C; Do not freeze!
Stability:	6 months after shipping
Shipping:	Blue ice