- 询价
- IBA
- 德国
- 2-1000-002
- 2026年01月04日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4-8℃
- 保质期：
6
- 英文名：
Desthiobiotin
- 库存：
999+
- 供应商：
IBA Lifesciences
- 规格：
1 g（2-1000-002）/5 g（2-1000-005）
| 规格： | 1 g（2-1000-002） | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 5 g（2-1000-005） | 产品价格： | 询价 |
Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin® in a competitive manner, resulting in a mild elution. Desthiobiotin powder can be used for preparation of Strep-Tactin® elution buffer, Buffer E. Please note that desthiobiotin is not applicable for elution from Strep-Tactin®XT resins.
Specifications
| Alternative Names: | 5-Methyl-2-oxo-4-imidazolidinehexanoic acid |
|---|---|
| Form: | Powder |
| Molecular Weight: | 214.3 g/mol |
| Possible Application: | Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins |
Shipping information
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验[1] Grun A F R, Said F A, Schamoni-Kast K, et al. Fast-tracking native mass spectrometry: Skipping over buffer exchange[J]. bioRxiv, 2025: 2025.02. 22.639503.
[2] Dróżdż M, Zuvanov L, Sasikumar G, et al. Immediate early splicing controls translation in activated T-cells and is mediated by hnRNPC2 phosphorylation[J]. The EMBO Journal, 2025: 1-32.
[3] Zhang X, Lu L, Yi C, et al. Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq[J]. STAR protocols, 2025, 6(1): 103599.
[4] Porter R S, An S, Gavilan M C, et al. Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex[J]. Cell Reports, 2025, 44(1).
下，经过快速的洗脱步骤，得到高纯度、高浓度的目标蛋白 (> 95%) 。 Strep-Tactin® 与 Strep-Tactin®XT 纯化流程的差异主要在于： 将靶蛋白从 Strep-Tactin®XT 洗脱时，需使用含有 50 mM Biotin (生物素) 的 Buffer BXT (由于亲和力大幅提升，Desthiobiotin 不能有效的洗脱靶蛋白。) 再生 Strep-Tactin®XT Superflow® 填料时，需使用 10 mM NaOH (因使用生物素进行洗脱
指具有维生素作用和活性的衍生物。如视黄醇酯、视黄醛、视黄酸是维生素 A的同效维生素，而脱硫生物素（ desthiobiotin）在许多细菌中是生物素的同效维生素。
Purification and Analysis of Strep-tagged Antibody-Fragments
. The fact that the interaction between the Strep-tag and streptavidin is highly specific and can be reversed upon addition of biotin (or suitable derivatives such as desthiobiotin) enables a highly efficient affinity chromatography under biochemically mild
技术资料暂无技术资料 索取技术资料
