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文献和实验(called a preincubation assay) has been the most frequently used. In this assay, dimethyl sulfoxide (DMSO) has been often used at a concentration of 7 or 14 % (particularly in Japan) in the preincubation mixture (0.05 or 0.1 mL of DMSO in 0.7 mL of the mixture
during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation. 生物学中的应用 : DMSO is used in PCR to inhibit secondary structures in the DNA template or the DNA primers
? ylhuang0502 I have never had this problem when I used LY 294002. "as an alternative to DMSO try methanol (solubility up to ~1 mg/ml); if it meets your concentration for use in cell culture it is to prefer instead of DMSO
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