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- 详细信息
- 文献和实验
- 技术资料
- 库存:
18
- 英文名:
Poly(A) Polymerase (E.coli)
- 保质期:
1年
- 供应商:
上海泽叶生物科技有限公司
- 保存条件:
-20
- 规格:
100U/1,000U
| PolyA 聚合酶为高效加A聚合酶,该酶以RNA为模板在RNA的3′末端加入20~200个A碱基。可应用于增强mRNA的稳定性,及microRNA加A尾,为cDNA合成提供oligo-dT引物结合位点等。海基生产的PolyA聚合酶是E.coli来源的,该酶是以单链RNA 作为模板,ATP作为底物进行聚合反应的。 | ||||||||||||
组分 |
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来源 |
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| 重组表达的E.coli来源的Poly(A)聚合酶。 | ||||||||||||
单位定义 |
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| 在37℃、pH7.9 的条件下,以ATP 为底物,10 分钟内把1 nmol的AMP 聚合到RNA上所需要的酶量定义为1个活性单位(U)。 | ||||||||||||
应用 |
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储存 |
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| -20°C可保存2年。 | ||||||||||||
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文献和实验Activity Assays for Poly-ADP Ribose Polymerase
Poly(ADP-ribose) polymerase (PARP-1) is a nuclear enzyme that has traditionally been thought to require discontinuous or “damaged” DNA (dcDNA) as a coenzyme, a preconception that has limited research mainly to its role in cell pathology, i.e
Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage
Poly (ADP-ribose) polymerase (PARP-1; EC 2.4.2.30) is a predominantly nuclear enzyme long presumed to function in the identification and repair of DNA strand nicks and breaks (for recent reviews see: 1 –3 ). PARP is comprised of a N
, the proteins were solubilized with different methods, and the affinity chromatography on NAD-Agarose of the first protocol was replaced by a gel filtration step in the second protocol. When enzyme activity was compared with electrophoresis and anti-poly-ADP
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