- ￥3890.16
- BioCoat
- 354257
- 2025年11月17日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
Corning
- 规格：
Advanced Cells
Source
Bovine Articular Cartilage
Quality
Bovine Collagen Type II is a membrane filtered (0.2um) preparation.
>95% by SDS-PAGE
Found negative for bacteria and fungi.
Preparation and Storage
Stable for a minimum of 3 months from date of shipment when stored at -70°C.
Handling
Used for attachment and differentiation of chondrocytes
Used as a thin coating on tissue culture plastic.
We recommend coating at 5 µg per cm 2 Please use this as a guideline for determining the optimum concentration for your application.
Formulation
As a liquid in 15Mm Acetic Acid
Details
| Product Number | 354257 |
| Qty./Pk | 1 / Pk |
| Qty./Cs | 1 / Ea |
| Brand | Corning® |
| Size | 5 mg |
| Source | Bovine |
| Formulation | As a liquid in 15Mm Acetic Acid |
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文献和实验E.Z.N.A.® Protocol for Bacteria
/lysozyme and incubate at RT for 7 min. Centrifuge 1010 cells at 4,000 x g for 5 min. Discard supernatant and add 100 ul of TE buffer containing lysozyme (0.5 mg/ml for Gram-negative and 4 mg/ml for Gram-positive bacteria). Resuspend cells completely
V294.PART II，Chapter 2 Boyden法体外趋化试验
9791; Sigma-Aldrich): dissolve collagen at 1mg/mL in 0.1 N acetic acid. Allow to stir at room temperature until dissolved(takes 1–3 h). Keep collagen stock at 4°C. 9. Methanol. 10. Giemsa stain, modified solution (cat. no. GS500; Sigma-Aldrich
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
A. 6. Add 50mg glass beads and vortex at maxi speed for 5 minutes. Let it stand to allow the beads to settle. Transfer the supernatant to a new 1.5 ml centrifuge tube (not supplied). 7. Add 250 ul YP II and gently mix by inverting and rotating
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