- ￥2321.75
- BioCoat
- 354231
- 2025年11月17日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
Corning
- 规格：
Advanced Cells
Source
Bovine dermis
Quality
Purity > 95% by SDS-PAGE
Shown to promote attachment and spreading of HT-1080 human fibrosarcoma cells
Tested for formation of a firm gel by exposure to ammonia vapors from solutions of 0.5 mg/ mL. Further dilution will decrease the rigidity of the gel. NOTE: This preparation contains native collagen molecules with a small amount of nicked or shortened sequences due to pepsin treatment.
Tested and found negative for bacteria, fungi, and mycoplasma
Formulation
Liquid in 0.012 N HCl
Preparation and Storage
Stable for at least three months at 2° to 8°C. Do not freeze.
Handling
Effective as a gel or thin coating at a recommended concentration of 5-10 µg/cm² of growth surface depending on cell type
Details
| Product Number | 354231 |
| Qty./Pk | 1 / Pk |
| Qty./Cs | 1 / Ea |
| Brand | Corning® |
| Size | 30 mg |
| Source | Bovine |
| Formulation | Liquid in 0.012 N HCl |
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文献和实验V294.PART II，Chapter 2 Boyden法体外趋化试验
. If a soluble attractant suchas growth factor is used, the ECM protein should still be loaded to allow cellsto attach. Collagen and fibronectin are usually chosen respectively for epithelialcells and fibroblasts to attach. The following protocol is intended
once with deionized water. 2) Stain gel in 0.006% Coomassie Brilliant Blue R-250 in 10% acetic acid for 1-6 hrs. (0.006% = 30mg in 500mls) 3) Destain in 10% acetic acid 2-12hrs. If necessary, gel can be stored in 10% acetic acid at 4OC for up to 1wk until ready
V294.PART II，Chapter 5 细胞粘附实验（英文版）
variations to thebasic protocols and areas where special attentions are required for successfulapplication of the methods. 2. Materials 2.1. Static Adhesion Assays 1. 96-Well enzyme-linked immunosorbent assay (ELISA) plates (Corning
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