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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海研卉生物科技有限公司
- 库存:
50ul
- 靶点:
AKT
- 级别:
科研
- 目录编号:
10176-2-AP
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 抗体英文名:
AKT Polyclonal antibody
- 抗体名:
Akt抗体
- 标记物:
无
- 宿主:
Rabbit
- 适应物种:
human, mouse, rat
- 亚型:
Rabbit / IgG
- 形态:
液体
- 应用范围:
FC, IF, IHC, IP, WB, ELISA
- 浓度:
600 μg/ml
- 规格:
100ul
AKT Polyclonal antibody
AKT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
1033
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, mouse brain tissue |
| Positive FC detected in | HeLa cells |
Cat No : 10176-2-AP
Synonyms
AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
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Cell Res RAB31 marks and controls an ESCRT-independent exosome pathway.Authors - Denghui Wei | ||
|
Nat Cell Biol Heat stress activates YAP/TAZ to induce the heat shock transcriptome.Authors - Min Luo | ||
|
Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer riskAuthors - Pingting Ying | ||
|
Sci Adv Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.Authors - Patrick Han | ||
|
Acta Pharm Sin B Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatmentAuthors - Lin Hou | ||
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Brain MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.Authors - Bing-Lin Zhu |
Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人磷酸化AKT 蛋白 ( AKT )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 AKT 单抗包被于酶标板上,标准品和样品中的 AKT与单抗结合,加入生物素化的抗人 AKT ,形成免疫
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠磷酸化 AKT 蛋白 (AKT)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 AKT 单抗包被于酶标板上,标准品和样品中的 AKT 与单抗结合,加入生物素化的抗大鼠 AKT ,形成免疫复合物连接在板上
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