SOLUBILIZATION BUFFER IBLYSI

Quantifying Protein in 2-D PAGE Solubilization Buffers

or loading extract from the same number of cells is inaccurate. The author has observed that radioactively labeled cell extracts exhibit 10–20% variances in protein concentration. Similarly, solubilization of 2�106 cells from a number of colorectal carcinoma

Restriction Enzyme Buffer

Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard

ChIP Protocol-Mechanical Breakage & FA Lysis Buffer

ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day