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- 2025年10月01日
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文献和实验Electrotransfection with Intracellular Buffer
is electroporation (for reviews, see refs. 1 ,2 ). Compared to other techniques for the introduction of molecules into living cells, electroporation does not seem to cause prolonged alterations in the biological structure and function of the target cells
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
Transformation of E. coli by Electroporation
dry the pellet. 6. Resuspend the DNA in 0.5X TE buffer [5 mM Tris-HCl, 0.5 mM EDTA (pH 7.5)] to a concentration of 10 ng/ul of DNA. Use 1 μl per transformation of 20 μl of cell suspension. III. Electroporation. 1. Mark the required
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