- 询价
- 07-690
- 2025年07月13日
- Chromatin Immunoprecipitation
- Rabbit
- Chicken;Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Chromatin Immunoprecipitation
- 宿主：
Rabbit
- 库存：
大量
- 适应物种：
Chicken;Human;Mouse;Rat
- 抗原来源：
0
- 是否单克隆：
2
- 规格：
100 μL
Product Images
Acid extracted proteins from HeLa cells untreated (lane 1) or treated with sodium butyrate (lane 2) or colcemid (lane 3) and recombinant Histone H3 (l...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, R, Yeast (S. cerevisiae ) | Chromatin Immunoprecipitation (ChIP), WB | Rabbit | Serum | Polyclonal Antibody |
- H3 histone family, member T
- histone 3, H3
- histone cluster 3, H3
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
- Chicken
- Human
- Mouse
- Rat
- Yeast (S. cerevisiae )
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
- HIST3H3
- H3FT
- MGC126886
- H3t
- MGC126888
- H3T
- H3/g
- H3.4
- H3/t
Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Product Family Information
Histone H3Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3 详细描述见链接：http://www.millipore.com/catalogue/item/07-690
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文献和实验in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
is lost on tips). Rotate in cold room for 1 hr. Spin and wash beads twice with RIPA (discard supernatant), and then once with Histone Wash Buffer. Add 30 µL of Histone Assay solution. Incubate at 37°C for exactly 30 min. Mix
人抗狂犬病毒抗体(anti-RV)酶联免疫分析（ELISA）
标准品 100μl ，然后在第一、第二孔中加标准品稀释液 50μl ，混匀；然后从第一孔、第二孔中各取 100μl 分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液 50μl ，混匀；然后在第三孔和第四孔中先各取 50μl 弃掉，再各取 50μl 分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液 50ul ，混匀；混匀后从第五、第六孔中各取 50μl 分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液 50μl ，混匀后从第七、第八孔中分别取 50μl 加到第九、第十孔中
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