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文献和实验is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
Purification of TFIIA from E. coli
.A protein assay is done,then the two subunits are mixed together in equimolar amounts at 0.4 � 1 mg/ml (additional RB+ 7M urea is added to obtain correct concentration for renaturation).The two subunits are then renatured together during dialysis against RB
Purification of TFIIA from E. coli
Purification of TFIIA from E.coli Linda Warfield,Hahn Lab,2003 Volumes given are for 2L of cells for each subunit (Toa1 and Toa2). Toa1 and Toa2 subunits are expressed separately in cultures of OD0.6 for 2 hours with 0.4mM IPTG at 37 degrees
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