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文献和实验Phagocytic leukocytes (granulocytes, monocytes, and macrophages) provide a first line of host defense by their ingestion (phagocytosis) and killing of microorganisms. Leukocytes phagocytize particles through a complex series of events
In-vitro Phagocytosis Assay of Macrophages
of the phagocytosis assay is simple. Briefly, phagocytic (antigenic; yeast in this case) cells are incubated with the macrophages. The cells were stained with May-Grunwald stain, and attached and engulfed cells were counted with the help of microscope. Reagents
2 mg TRITC, incubate 30 minutes at 37 ° C on a rotary shaker. Wash 2 x in Na2 HPO4 (50 mM, pH 9.2) and 4 x in PB. Freeze aliquots of 109 particles/ml at -20 ° C. Phagocytosis assay
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