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文献和实验Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Dehydrogenase (LDH) Measurement
In order to improve therapeutic antibodies efficacy in cancer patients, several strategies were developed. One of these strategies consists in the enhancement of effector functions. Antibody-dependent cellular cytotoxicity (ADCC) was shown
Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity
polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput screening
/ml IL-2 and a gradually weaned to 50 U/ml IL-2. The evening before the assay (day -1 ), passage the cells from 50 U/ml IL-2 into the desired level of IL-2 for overnight (16h) stimulation. Occasionally, IL-2 levels > 50 U/ml are necessary to maintain
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