Cyclic AMP Select ELISA Kit

腺苷酸环化酶3',5'-单磷酸（cAMP）是一种普遍存在于细胞内的第二信使，是连接膜受体及其配体与细胞内酶活性和基因表达激活的关键信号转导通路的组成部分。cAMP由膜结合的腺苷酸环化酶从ATP合成。配体或激素与其特定的G蛋白偶联受体（GPCRs）结合后，会激活GTP结合蛋白（Gs或Gi），这些蛋白可以刺激或抑制腺苷酸环化酶。cAMP通过促进酶的磷酸化或去磷酸化来激活或抑制各种酶或一系列酶。cAMP信号通过磷酸二酯酶将cAMP水解为AMP而被中和。因此，细胞内cAMP的浓度是腺苷酸环化酶从ATP合成cAMP的速率与特定磷酸二酯酶将其分解为AMP的速率的函数。Cayman的cAMP选择性检测是一种竞争性酶联免疫吸附测定（EIA），可在标准曲线范围内（0.09-200pmol/ml）进行cAMP测量，灵敏度（80% B/B₀）约为0.6pmol/ml。在这种高灵敏度的检测中，无需对样本进行乙酰化处理。Adenosine 3',5' cyclic monophosphate (cAMP) is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of ligands or hormones to their specific GPCRs activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases. Cayman's cAMP Select assay is a competitive EIA that permits cAMP measurements within the standard curve range of 0.09-200 pmol/ml and a sensitivity (80% B/B₀) of approximately 0.6 pmol/ml. Acetylation of samples is not required in this highly sensitive assay.分子式分子量