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文献和实验for understanding their role in chromatin structure and regulation of DNA functions. Here are described three new protocols for histone isolation from undisturbed whole cells. They avoid the conventional non-denaturing cell lysis, which affects the native
Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
of the 601 DNA array with the Histone octamer and Linker histones whilst preventing oversaturation (Huynh et al, 2005; Robinson et al, 2006; Routh et al, 2008). The assembly of the nucleosome arrays is by the classical and simple salt reconstitution
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