- 询价
- 07-436
- 2025年08月09日
- Chromatin Immunoprecipitation
- Rabbit
- Human;Vertebrates
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Chromatin Immunoprecipitation
- 宿主：
Rabbit
- 库存：
大量
- 适应物种：
Human;Vertebrates
- 抗原来源：
0
- 是否单克隆：
2
- 规格：
200 μg
Product Images
WB:HeLa acid extract (lane 1 and lanes 3 through 7) and recombinant Histone H3 (lane 2) were probed with anti-monomethyl- Histone H3 (Lys4) (0.5μg/ml;...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Vrt | IC, IF, PIA, WB | Rabbit | Purified | Polyclonal Antibody |
- H3 histone family, member T
- histone 3, H3
- histone cluster 3, H3
- Immunocytochemistry
- Immunofluorescence
- Peptide Inhibition Assay
- Western Blotting
Reported by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
- Human
- Vertebrates
- HIST3H3
- H3FT
- H3/t
- H3t
- MGC126888
- H3/g
- H3.4
- H3T
- MGC126886
Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
Product Family Information
Histone H3Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
KLH-conjugated, synthetic peptide containing the sequence ARTmeK, corresponding to monomethyl-lysine at residue 4 of human histone H3 详细描述见链接：http://www.millipore.com/catalogue/item/07-436
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文献和实验upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
Installation of Site‐Specific Methylation into Histones Using Methyl Lysine Analogs
:12033‐12038. Kang, T.J., Yuzawa, S., and Suga, H. 2008. Expression of histone H3 tails with combinatorial lysine modifications under the reprogrammed genetic code
1 2 3 4 5 6 7 8 9 10 11 牛血清白蛋白标准品（ml） 0 0.2 0.2 0.4 0.1 0.6 0.6 0.8 0.8 1.0 1.0 生理盐水（ml） 1.0 0.8 0.8 0.6 0.6 0.4 0.4 0.2 0.2 0 0 蛋白含量(μg／ml) 0 50 50 100 100 150 150 200 200 250 250⑶ 每管加试剂甲5ml，混合后室温放置10min，再加0.50ml试剂乙（即Folin酚试剂），立即摇匀（否则显色降低）。⑷ 30min后测OD650nm。⑸ 以OD为纵坐标
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