- 询价
- 07-448
- 2025年08月09日
- Western Blotting
- Rabbit
- Vertebrates
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting
- 宿主：
Rabbit
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Vertebrates
- 是否单克隆：
2
- 规格：
200 μg
Product Images
Acid extracts from HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-monomethyl Histone H3 (Lys 27) (0.2...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Vrt | DB, IC, WB | Rabbit | Purified | Polyclonal Antibody |
- H3 histone family, member T
- histone 3, H3
- histone cluster 3, H3
- Dot Blot
- Immunocytochemistry
- Western Blotting
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
- Human
- Vertebrates
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
- HIST3H3
- H3FT
- H3/t
- H3t
- MGC126888
- H3/g
- H3.4
- H3T
- MGC126886
Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.
Product Family Information
Histone H3Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Synthetic 2X-branched peptide containing the sequence ..ARmeKSA.. in which meK corresponds to monomethyl-lysine at residue 27 of human Histone H3. 详细描述见链接：http://www.millipore.com/catalogue/item/07-448
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文献和实验显微镜 日本奥林巴斯光学仪器有限公司研制的CK-2。 1.2. 方法 1.2.1摸拟DNA提取 取EDTA抗凝静脉血0.3ml 采用快速酚氯仿抽提DNA。 1.2.2PCR扩增PCR反应体至25μl，其中B27引物2μmol/L， 内对照物1μmol/L,Mgcl2 2mmol/L 17.5mmol/Ltric,Kcl37.5mmol/L,DNTP200μmol/L,5%Dmso,Taq酶0.5μ模板DNA22.5μl。 1.2.3扩增条件 95摄氏度变性5分钟，然后95摄氏
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
should also be included. In Ringrose et al ., (2004) I used histone H3 peptides spanning residues 1-13 (for lysine 4 and lysine 9 modifications) or 21-33 (for lysine 27 modifications). Stock solutions of 0.5mM peptide in PBS can be prepared and kept at -20°C
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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