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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Western Blotting
- 宿主:
Rabbit
- 适应物种:
Human;Mouse
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
2
- 规格:
200 μL
Product Images
WB: Jurkat cell lysate was probed with Anti-dimethyl-Arginine, asymmetric (ASYM24), (1:500). Several proteins containing asymmetrically dimethylated ...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rabbit | Serum | Polyclonal Antibody |
- Human
- Mouse
Included Positive Antigen Control:
Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Western Blot Analysis:
A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
peptide containing asymmetric dimethyl-arginine-glycine repeats 详细描述见链接:http://www.millipore.com/catalogue/item/07-414
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文献和实验Purification of recombinant sBRF M166L
300mM NaCl 2.63 g NaCl 6.0M Gu-HCl 86 g Guanidine-HCl 10mM Imidazole 0.1 g Imidazole Wash Buffer, pH8.0 (200 ml
Purification of recombinant sBRF M166L
-HCl 10mM Imidazole 0.1 g Imidazole Wash Buffer,pH8.0 (200 ml) 30 mM Tris 8.0 0.73 g Tris 300mM NaCl3.5 g NaCl 8.0M Urea 96.2 g Urea 10mM Imidazole 0.136 g Imidazole H2O to 200 ml final Elution Buffer,pH 8.0 (50 ml) 20mM Tris 8.0 0.12 g
Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism
‐mm GSWP filter microfiltration tubes, sterile (Ultrafree, GV Durapore; Millipore) HPLC autosampler injection vials with 200‐μl inserts
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