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文献和实验Site-directed hydroxy radical mapping of nucleosome positions in vitro
12). Mapping Reaction A typical sample for mapping has a total volume of 10-20µl and contains 0.2-0.5µM reconstituted nucleosome, 50mM Tris-Cl pH 7.5 and appropriate monovalent salt. After desired experimental manipulation, place sample
Rapid Determination of Glutamate Using HPLC Technology
. The ionotropic receptors are comprised of three families with intrinsic cation permeable channels (Na+ and K+ ): N-methyl-D-aspartate (NMDA), 2-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and kainate. The latter two families are known as the nonNMDA
Calcien Blue 370 435 Calcium Green 505 532 Calcium Orange 549 576 Calcofluor RW Solution 370 440 Calcofluor White 440 500-520 Calcofluor WhiteABT Solution 380 475 Calcofluor WhiteStandard Solution 365 435 5-(and 6-)carboxySNARF-1 indicator 548(low
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