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文献和实验Selection of Non-aggregating VH Binders from Synthetic VH Phage-Display Libraries
The particular interest in VH antibody fragments stems from the fact that they can rival their “naturally occurring” single-domain antibody (sdAb) counterparts (camelid VH Hs and shark VNAR s) with regard to such desirable characteristics
构建人VH基因节段文库 [器材和试剂] ● PCR试剂和设备 ● 自pHENl中天然scFv文库制备的scFv基因文库模板DNA（单链和双链DNA 10ng/ul） ● Genecle&n试剂盒（Qbiogene） ● PVH3FoR1引物： 5'-TCG CGC GCA GTA ATA CAC GGC CGT GTC-3' ● PVH3FORl引物： 5'-TCG CGC GCA GTA ATA CAC AGC CGT GTC CTC-3' ● PVlJ
Construction of a Human Antibody Domain (VH) Library
) or the complementarity-determining regions (CDRs) of the variable domains of antibodies. In the late 1980s, the smallest known antigen-binding fragment was identified when a murine VH repertoire was screened for binding to lysozyme. This fragment (∼15 kDa
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