SF1670

Native purification of His-tagged Proteins from Sf9 cells

Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0 1 % NP-40      1 mM PMSF      1 µg/ml leupeptin 3. Transfer to oakridge tubes and sonicate

GPCR Expression Using Baculovirus-Infected Sf9 Cells

promoter-driven expression of a C-terminal 6�histidine-tagged mammalian M2 muscarinic receptor in Sf 9 cells. Results demonstrate that expressed receptor could be detected and quantified using radiolabeled ligand binding, that expression was maximal

Purification of Recombinant p53 from Sf9 Insect Cells

We describe a method for purifying recombinant p53 from baculovirus infected cells in one step by anion exchange chromatography. The p53 is full-length with no flanking sequences and its expression is driven by the baculovirus polyhedron