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文献和实验Cytogenetic Characterization of Recombinant Cells
The use of mammalian cell systems for recombinant protein production has become the dominant method to produce high-value proteins for therapeutic purposes. Recombinant cell lines are often generated by transfection with specific DNA
Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells
The purpose of this chapter is to propose a practical procedure for the generation and selection of Chinese hamster ovary cells producing high levels of recombinant protein by combining in vitro and in vivo amplification of the foreign gene
Human Prostate Cancer Cell Lines
complex. Growth of early prostate cancer requires 5α-dihydrotestosterone produced from testosterone by the 5α-reductase enzyme system; such prostate cells are described as androgen dependent (AD). Subsequently, the prostate cancer cells may respond
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