JC-1 Mitochondrial Membrane Po

线粒体膜电位（ΔψM）是线粒体功能的重要参数，可作为细胞健康的指示物。JC-1是一种亲脂性阳离子染料，可以选择性进入线粒体，并随着膜电位的增加，反向改变颜色，从绿色变为红色。在具有高线粒体膜电位（ΔψM）的健康细胞中，JC-1自发地形成称为J-聚集体的复合物，显示出强烈的红色荧光。另一方面，在具有低膜电位（ΔψM）的凋亡或不健康细胞中，JC-1保持在单体形式，仅显示绿色荧光。Cayman的JC-1线粒体膜电位检测试剂盒提供所有必要的试剂及完整的操作说明，用于分析全细胞中的线粒体完整性。该检测试剂盒适用于荧光显微镜和荧光酶标仪。对于流式细胞术，推荐使用Cayman的JC-1线粒体膜电位流式细胞术检测试剂盒（Cayman_701560）。Mitochondrial membrane potential, (ΔψM), is an important parameter of mitochondrial function used as an indicator of cell health. JC-1 is a lipophilic, cationic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial (ΔψM), JC-1 spontaneously forms complexes known as J-aggregates with intense red fluorescence. On the other hand, in apoptotic or unhealthy cells with low (ΔψM), JC-1 remains in the monomeric form, which shows only green fluorescence. Cayman's JC-1 Mitochondrial Membrane Potential Assay Kit provides all the necessary reagents, as well as complete instructions, for analysis of mitochondrial integrity in whole cells. The assay is applicable to fluorescence microscopy and fluorescent plate reader. For flow cytometry, Cayman's JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit (Cayman_701560) is recommended.