• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Measurement of Mitochondrial Membrane Potential and Proton Leak

        互联网

        572
        The major component of mitochondrial electrochemical potential gradient of protons is the mitochondrial membrane potential (ΔΨ ), and hence it is a suitable parameter for assessment of mitochondrial function. Dissipation of the mitochondrial membrane potential causes uncoupling of the electron transport through the respiratory chain and the phosphorylation reaction for ATP synthesis (proton leak). Proton leak functions as a regulator of mitochondrial reactive oxygen species (ROS) production and its modulation by uncoupling proteins, which may be involved in pathophysiology. In this report, we describe the assays for mitochondrial membrane potential and proton leak, which require a TPP+ electrode and a Clark electrode. The determination of mitochondrial peroxide production with homovanillic acid is also included.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序