NF-κB (p65) Transcription Fact

NF-κB/Rel家族的转录因子由几种结构相关的蛋白质组成，它们可以形成同源二聚体和异源二聚体，包括p50/p105、p52/p100、RelA(p65)和c-Rel/NF-κB。作为二聚体，这些转录因子能够结合到DNA上的κB位点，从而调控目标基因的表达。在哺乳动物中最普遍的Rel/NF-κB二聚体包含p50-RelA(p50/p65)异源二聚体，它是治疗人类炎症和某些其他疾病的潜在治疗靶点。Cayman的NF-κB(p65)转录因子检测试剂盒是一种非放射性、灵敏的方法，用于检测核提取物和全细胞裂解物中人类NF-κB(p65)的DNA结合活性。它不会与NF-κB(p50)发生交叉反应。The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-κB. Acting as dimers, these transcription factors bind to κB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-κB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman's NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting human NF-κB (p65) DNA binding activity in nuclear extracts and whole cell lysates. It will not cross-react with NF-κB (p50).