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文献和实验The activation of CD4+ T-cell subsets, Th1 and Th2, is one of the most important focuses of immunology. Th1 and Th2 are differentiated from naive T cells after antigenic stimulation by the influence of cytokines [interleukin 12 (IL
Mag-Bind® mRNA Protocol (Standard Protocol)
capable of 12,000 x g and 2-8°C 实验步骤 Total RNA Isolation: 1. Homogenization and lysis of samples: follow either method below. 1) Tissue Samples Homogenize tissue samples in 1.5 mL of RNA-Solv ® Reagent per 100
RNASafer® Protocol for Total RNA Isolation
buffer. Homogenize the tissue with a rotor-stator homogenizer. For RNA isolation using Midi and Maxiprep kit, follow the standard protocol in the user manual. 4) Follow standard protocol for E.Z.N.A.® Total RNA Kits in user manuals.
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