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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 应用范围:
Chromatin Immunoprecipitation
- 宿主:
Rabbit
- 适应物种:
Eukaryote
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
2
- 规格:
200 μL
WB: 10 micrograms of acid extracts of untreated (lane 1) or butyrate-treated (lane 2) HeLa cells probed with Anti-acetyl Histone H4 at a 1:2000 dilution.
Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of HeLa and NIH/3T3 cells using 06-866 (Red). Actin filaments have been labeled with AlexaFluor®488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 µL of either a normal rabbit antiserum or Anti-Acetyl Histone H4 serum and the Magna ChIP A (Cat. # 06-866) Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter.
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, T, Eu | Chromatin Immunoprecipitation (ChIP), WB, IC | Rabbit | Serum | Polyclonal Antibody |
- H4 histone family, member A
- histone 1, H4a
- histone cluster 1, H4a
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
- Immunocytochemistry
5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
- Human
- Tetrahymena
- Eukaryote
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- HIST1H4A
- H4/J
- HIST1H4F
- HIST1H4L
- H4FN
- H4FA
- H4FH
- HIST1H4H
- H4F2
- H4FM
- H4/A
- H4FK
- H4FG
- HIST2H4
- H4FB
- H4/K
- HIST1H4I
- H4/N
- HIST1H4B
- H4FE
- H4/M
- H4/a
- IST1H4E
- HIST4H4
- HIST2H4A
- H4FC
- H4FI
- H4/H
- HIST1H4C
- H4/G
- HIST1H4K
- 4FJ
- H4FD
- H4/I
- H4/B
- H4/D
- H4/C
- HIST1H4J
- HIST1H4D
- H4/E
Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
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peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16. 详细描述见链接:http://www.millipore.com/catalogue/item/06-866
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