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Anti-acetyl-Histone H4; 200 μL

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  • 询价
  • 06-866
  • 2025年07月15日
  • Chromatin Immunoprecipitation
  • Rabbit
  • Eukaryote
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 应用范围

      Chromatin Immunoprecipitation

    • 宿主

      Rabbit

    • 适应物种

      Eukaryote

    • 抗原来源

      0

    • 库存

      大量

    • 是否单克隆

      2

    • 规格

      200 μL

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    WB: 10 micrograms of acid extracts of untreated (lane 1) or butyrate-treated (lane 2) HeLa cells probed with Anti-acetyl Histone H4 at a 1:2000 dilution.

    Immunocytochemistry:
    Representative lot data.
    Confocal fluorescent analysis of HeLa and NIH/3T3 cells using 06-866 (Red). Actin filaments have been labeled with AlexaFluor®488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.

    Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 µL of either a normal rabbit antiserum or Anti-Acetyl Histone H4 serum and the Magna ChIP A (Cat. # 06-866) Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter.

    Species Reactivity Key Applications Host Format Antibody Type
    H, T, Eu Chromatin Immunoprecipitation (ChIP), WB, IC Rabbit Serum Polyclonal Antibody
    Description:
    Anti-acetyl-Histone H4
    Background Information:
    Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
    Alternate Names:
    • H4 histone family, member A
    • histone 1, H4a
    • histone cluster 1, H4a
    Specificity:
    Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
    Key Applications:
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    • Immunocytochemistry
    Application Notes:
    Chromatin Immunoprecipitation:
    5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
    Species Reactivity:
    • Human
    • Tetrahymena
    • Eukaryote
    Species Reactivity Note:
    Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
    Isotype:
    IgG
    Immunogen:
    KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
    Epitope:
    a.a. 2-19
    Modifications:
    Acetylation
    Molecular Weight:
    10 kDa
    Control:
    TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
    Presentation:
    Whole antiserum containing 0.05% sodium azide.
    Storage Conditions:
    Stable for 1 year at -20°C from date of receipt.
    Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    Gene Symbol:
    • HIST1H4A
    • H4/J
    • HIST1H4F
    • HIST1H4L
    • H4FN
    • H4FA
    • H4FH
    • HIST1H4H
    • H4F2
    • H4FM
    • H4/A
    • H4FK
    • H4FG
    • HIST2H4
    • H4FB
    • H4/K
    • HIST1H4I
    • H4/N
    • HIST1H4B
    • H4FE
    • H4/M
    • H4/a
    • IST1H4E
    • HIST4H4
    • HIST2H4A
    • H4FC
    • H4FI
    • H4/H
    • HIST1H4C
    • H4/G
    • HIST1H4K
    • 4FJ
    • H4FD
    • H4/I
    • H4/B
    • H4/D
    • H4/C
    • HIST1H4J
    • HIST1H4D
    • H4/E
    Quality Assurance:
    Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

    Western Blot Analysis:
    1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
    Antibody Category:
    Epigenetics & Nuclear Function
    Antibody Sub-Category:
    Histones
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Concentration:
    Variable
    Antibody Type:
    Polyclonal Antibody
    Format:
    Serum
    Host:
    Rabbit

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16. 详细描述见链接:http://www.millipore.com/catalogue/item/06-866

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