Anti-phospho-Histone H3 (Ser10

), Mitosis Marker; 200 μg

价格询价

货号06-570
更新日期2026年08月07日

应用范围Flow Cytometry;Immunoprecipitation
宿主Rabbit
适应物种Drosophila;Vertebrates

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详细信息
文献和实验
技术资料

应用范围：
Flow Cytometry;Immunoprecipitation
宿主：
Rabbit
库存：
大量
适应物种：
Drosophila;Vertebrates
抗原来源：
0
是否单克隆：
2
规格：
200 μg

Product Images

ICC: Asynchronously growing HeLa cells were stained with anti-phospho-Histone H3 (red) and anti-tubulin (green)

Western Blot Analysis
Acid extracts from untreated
(lane 1) and colcemid treated (lane 2)
HeLa cells were resolved by
electrophore...

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H	IC, BD, IP, WB	Rabbit	Purified	Polyclonal Antibody

Description:

Anti-phospho-Histone H3 (Ser10), Mitosis Marker

Background Information:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Alternate Names:

H3 histone, family 3A
H3 histone, family 3B
H3 histone, family 3B (H3.3B)

Specificity:

This antibody recognizes Histone H3 when phosphorylated at Ser10.

Key Applications:

Immunocytochemistry
Beadlyte® Assay
Immunoprecipitation
Western Blotting

Application Notes:

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Beadlyte® Assay Analysis: A representative lot recognizes Histone H3 phosphorylated on Ser10 by Luminex assay.

Species Reactivity:

Mouse
Human

Species Reactivity Note:

Demonstrated to react with human and mouse.

Immunogen:

Linear peptide corresponding to human Histone H3 at Ser10.

Epitope:

Ser10

Modifications:

Phosphorylation

Molecular Weight:

17 kDa

Control:

Colcemid treated HeLa acid extract

Presentation:

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.

Storage Conditions:

2-8°C for up to 6 months.

Gene Symbol:

H3.3A
H3.3B
H3F3
H3F3A

Quality Assurance:

Evaluated by Western Blot in Colcemid treated HeLa acid extract.
Western Blot Analysis: 0.5 – 1 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated HeLa acid extract.

Antibody Category:

Epigenetics & Nuclear Function

Antibody Sub-Category:

Histones

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Trade Name:

Upstate (Millipore)

Concentration:

1 mg/mL

Antibody Type:

Polyclonal Antibody

Format:

Purified

Host:

Rabbit

Product Family Information

Histone H3

Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.

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更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

peptide (ARKpSTGGKAPRKQLC) corresponding to amino acids 7-20 of human histone H3 详细描述见链接：http://www.millipore.com/catalogue/item/06-570

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文献和实验

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Anti-phospho-Histone H3 (Ser10), Mitosis Marker; 200 μg

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