- ￥4000
- ImmuneChem
- ICP0388
- 加拿大
- 2025年07月31日
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- 抗体英文名：
Acetyl Lysine Antibody, Agarose
- 宿主：
其它
- 抗原来源：
其它
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 是否单克隆：
0
- 规格：
2mg
Acetyl Lysine Antibody, Agarose 的详细介绍
- 类 型：各种亲和填料
Catalog NumberPack SizePrice(CNY)ICP03882 mg￥4000.00Product Description: The acetylated Lysine antibody is immobilized to the beaded agarose via amide linkage. The product could be utilized as affinity matrix for rapid isolation and purification of the species of protein or peptides with acetyl Lysine residues.Species: RabbitFormulation: 0.5 mL beaded agarose suspended in 1 mL of glycerol.Antibody immobilized: 4 mg/mL, antibody is covalently linked through amide bond with NHS activated-SMCC then linked to thiolated Agarose beads via thiol ether bond.Storage & Stability: Store product at –20 ℃. One year from date of shipping.Specificity: Selectively capture the peptides and proteins with acetylated lysine residues (N-epsilon). Not cross-react with methylated proteins and mono- and dimethylated proteins.Binding Capacity: Approximately 0.2 mg of acetylated histone/mL.Application(s): Immunoprecipitation, Immuno-Affinity Chromatography.Scientific Description: Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation, and identification of the acetylated protein/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid method to enrich and purify the acetylated species for further MS/MS identification.
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文献和实验Protocols for Lysine Conjugation
Currently, the most widely used chemical methodology for the conjugation of drugs to monoclonal antibodies involves either lysine or cysteine residues. In this chapter, several methods for the preparation of antibody–drug conjugates (ADCs
How Do I make an Antibody against a Peptides
-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids (glutamic, aspartic acid or 5'-phosphate) to the carrier protein. Keyhole limpet
make an Antibody against a Peptides
make an Antibody against a Peptides If your peptide is synthesized with a terminal cysteine residue (i.e., a free sulfhydryl group, -SH), you can use any maleimide-activated carrier protein
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